FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15103728
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC211400.0113.34674120
GAGCACA348300.064.793205120
AGAGCAC471300.049.504654118-119
CCGATCT148850.041.9986724
CGATCTA41550.038.2412455
TCCGATC175550.035.937033
CTTCCGA177000.035.9209631
AAGAGCA689000.033.23935118-119
GAAGAGC785850.029.778822116-117
TTCCGAT246150.026.1376172
GGAAGAG980450.023.430115116-117
CGGAAGA1164700.020.25055114-115
GATCTAG12650.020.1794246
TCGGAAG1192000.019.282894114-115
ATCGGAA1393400.016.986917112-113
GATCGGA1432200.016.12266112-113
AGATCGG1532700.015.428281110-111
CGATCTC104550.014.9485975
CACGTCT60350.014.818263120
TAGATCG428200.013.8316110-111
Produced by FastQC (version 0.11.5)