FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15228945
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187600.088.0629120
GAGCACA306650.055.70806120
AGAGCAC424000.041.54613120
AAGAGCA637150.027.526052118-119
GAAGAGC739050.023.655685118-119
GGAAGAG911750.019.30949116-117
CGGAAGA1065750.016.53664116-117
TCGGAAG1077850.016.363832120
ATCGGAA1247050.014.098376114-115
GATCGGA1264300.013.9215355112-113
AGATCGG1377800.012.737115112-113
TAGATCG359500.011.794781110-111
TTAGATC105300.011.382134120
CCGATCT72550.011.0023374
TCCGATC75450.010.652923
CTAGATC72350.010.534288114-115
GAGATCG467100.010.501819116-117
AAGATCG440850.010.203515120
CAGATCG444200.09.852287116-117
CGATCTA21500.09.7972695
Produced by FastQC (version 0.11.5)