FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17864485
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC182050.063.203255120
GAGCACA279900.042.77088120
AGAGCAC377450.032.658886120
AAGAGCA604000.021.532833120
GAAGAGC663450.019.32078120
GGAAGAG852000.015.902368120
TCGGAAG977800.013.689272118-119
CGGAAGA1010550.013.19628120
ATCGGAA1174600.011.89805120
GATCGGA1173500.011.793526120
AGATCGG1284900.011.435106120
TAGATCG288100.010.322145118-119
CTAGATC73550.09.603813118-119
CACGTCT68900.09.47572120
ACACGTC67850.08.860193120
AAGATCG586950.08.601219120
GAGATCG432500.08.534147120
CACACGT69950.08.501787120
TTAGATC123450.08.230214114-115
CAGATCG432750.07.997489116-117
Produced by FastQC (version 0.11.5)