FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13693303
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC186950.075.608536120
GAGCACA295800.048.04402120
AGAGCAC397950.035.667465118-119
AAGAGCA588350.025.01653120
GAAGAGC663500.021.795742120
GGAAGAG786150.019.049133120
CGGAAGA941250.015.672977118-119
TCGGAAG967500.015.323013118-119
ATCGGAA1128950.013.168195116-117
GATCGGA1151600.013.082471120
AGATCGG1280000.011.673665114-115
CACACGT46000.011.626923120
TAGATCG272550.010.827398114-115
CTAGATC71200.010.422724116-117
ACACGTC51600.010.230474120
CACGTCT53200.010.183917120
AAGATCG550650.09.641403114-115
TTAGATC115450.09.445775120
GAGATCG408900.09.091523118-119
CCTAGAT20700.09.046297116-117
Produced by FastQC (version 0.11.5)