FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008464
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187100.085.672806120
GAGCACA300350.054.027264120
AGAGCAC412250.041.797028120
AAGAGCA620100.027.253881118-119
GAAGAGC704200.024.020649118-119
GGAAGAG853350.019.845734116-117
CGGAAGA995250.017.054388116-117
TCGGAAG1006850.016.737637114-115
ATCGGAA1192450.014.339762114-115
GATCGGA1205100.014.056296112-113
AGATCGG1321150.012.974125112-113
CACACGT50950.012.685888120
TAGATCG274800.012.18999116-117
CTAGATC59450.011.6401415110-111
CACGTCT54300.011.203052120
ACACGTC55750.011.048068120
AAGATCG435000.010.418994110-111
GAGATCG464000.010.243086110-111
TTAGATC88450.010.230463120
CAGATCG499950.09.955415112-113
Produced by FastQC (version 0.11.5)