FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14256719
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC194550.079.567085120
GAGCACA323250.046.86395120
AGAGCAC424350.036.445602120
AAGAGCA619100.025.185688120
GAAGAGC722050.022.570078120
GGAAGAG845000.018.981855118-119
CGGAAGA999450.016.235228118-119
TCGGAAG1025000.015.644987116-117
ATCGGAA1200450.013.672285116-117
GATCGGA1241550.013.072167114-115
CACACGT50150.011.93672120
ACACGTC62000.011.927093120
AGATCGG1400250.011.804364114-115
TAGATCG262950.011.731077120
CTAGATC78650.010.476485112-113
CACGTCT54200.010.265138120
TTAGATC99800.09.879489120
ACGTCTG66100.09.69565120
AAGATCG532100.09.298011118-119
GAGATCG547300.08.8916235112-113
Produced by FastQC (version 0.11.5)