FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18107636
Sequences flagged as poor quality0
Sequence length53-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA3921650.016.20152120
TCATTAA1369600.010.356359120
TCTCGGG53450.010.2368555120
CATTAAA1305350.010.225838120
CGGTGGG41900.09.901658120
GTATCAT1754000.09.848701120
ATCATTA1513050.09.815594120
ATTAAAA1365050.09.796233120
TATCATT1712650.09.675725120
CGTATCA1870300.09.577059120
CCGTATC2059250.09.328991120
GCCGTAT2249200.09.097179120
GCGTCGG144000.08.852098120
CGCCGTA2430400.08.602008120
TTAAAAA1366200.08.595296120
TAAAAAA1376000.08.411728120
TCGCCGT2658900.08.021066120
GTAGGGG31500.08.016995120
GTCGCCG2818450.07.9830036120
GGTCGCC2911950.07.931097120
Produced by FastQC (version 0.11.5)