FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13302547
Sequences flagged as poor quality0
Sequence length58-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG239700.09.939773120
TAGATCT175000.09.670859120
AGAGTGT290650.09.66339120
GAGTGTA258000.09.351049120
GTGTAGA247100.08.962055120
AAGAGTG331200.08.951393120
TGTAGGG571450.08.769245120
TAGGGAA488950.08.56732120
GTGTAGG620150.08.506406120
CGTGTAG664650.08.424471120
GTAGGGA516550.08.202501120
GTAGATC262950.08.033846120
TCGTGTA706800.07.947551120
GGGAAAG502950.07.720288120
AGCGTCG954200.07.698321120
AAAGAGT396850.07.561321120
TCTCGGT163750.07.513228120
AGAGCGT1082400.07.512864120
AGGGAAA556200.07.455917120
AGATCTC192400.07.4549866120
Produced by FastQC (version 0.11.5)