FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15754453
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG251250.08.503347120
TAGATCT198350.08.441468120
GAGTGTA272650.08.144084120
AGAGTGT309300.08.129805120
TAGGGAA452800.08.00528120
TGTAGGG507600.07.673069120
GTAGGGA472750.07.284611118-119
CGTGTAG592400.07.2737120
AAGAGTG356650.07.2355614120
GTGTAGA269100.07.0249443120
GTAGATC276250.07.0169153120
GTCGTGT652400.06.9911017120
GTGTAGG564900.06.9585013120
AGCGTCG802900.06.8466797120
GTGGTCG143050.06.7124043120
TCGTGTA627250.06.649518120
CTCGGTG184100.06.4218245120
ATCTCGG196700.06.2545424120
TCTCGGT181650.06.244137120
GCGTCGT758050.06.2304997120
Produced by FastQC (version 0.11.5)