FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14679786
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163300.072.70993120
GAGCACA255850.043.920635120
AGAGCAC340100.034.22963120
AAGAGCA553000.021.698902120
GAAGAGC585950.020.28675118-119
GGAAGAG728000.016.737804120
CGGAAGA833900.014.858661120
TCGGAAG835700.014.632584118-119
ATCGGAA996300.012.609662120
GATCGGA1002300.012.263228118-119
AGATCGG1101450.011.496162120
TAGATCG298200.011.049258120
CTAGATC74950.09.729834120
CACACGT37750.09.658953120
CACGTCT44400.09.257486120
AAGATCG390150.09.108859116-117
ACACGTC39550.09.05173120
GAGATCG360100.08.773607114-115
CAGATCG373550.08.696243120
TTAGATC127550.08.576059120
Produced by FastQC (version 0.11.5)