FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14629675
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153350.071.28645120
GAGCACA261750.042.71794120
AGAGCAC339300.032.800682118-119
AAGAGCA546500.021.19335120
GAAGAGC608000.020.01128120
GGAAGAG734900.016.228184118-119
TCGGAAG840450.014.609485120
CGGAAGA840350.014.501774120
ATCGGAA994250.012.37764118-119
GATCGGA986500.012.328624118-119
AGATCGG1093750.011.586483120
CTAGATC79300.010.686955120
TAGATCG281600.010.363239112-113
GAGATCG420500.08.850845118-119
AAGATCG384050.08.726023116-117
TTAGATC113800.08.717103120
CACACGT40850.08.523564120
CAGATCG334450.08.289316120
TGAGATC180100.07.696729120
CACGTCT45550.07.3556194120
Produced by FastQC (version 0.11.5)