FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16203523
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC189100.077.43982120
GAGCACA294400.047.205757120
AGAGCAC410400.037.84088120
AAGAGCA633700.023.43241118-119
GAAGAGC679900.022.081278118-119
GGAAGAG873850.016.98905116-117
TCGGAAG996200.014.825901114-115
CGGAAGA1037600.014.768475116-117
ATCGGAA1199050.012.832655114-115
GATCGGA1203300.012.358518112-113
AGATCGG1293950.011.840493112-113
TAGATCG301400.010.740628112-113
CTAGATC70700.09.966826110-111
CACACGT51450.09.898763120
CACGTCT59400.09.86589120
ACACGTC57200.09.269601120
AAGATCG557150.09.040834120
GAGATCG444850.08.805685118-119
CAGATCG423200.08.308609120
TTAGATC123650.08.29405120
Produced by FastQC (version 0.11.5)