FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12781138
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123350.065.10775120
GAGCACA184350.044.433285120
AGAGCAC251050.035.46196120
AAGAGCA413200.022.445482120
GAAGAGC452400.020.316425120
GGAAGAG591700.016.140072120
TCGGAAG685400.013.868126120
CGGAAGA702950.013.442129118-119
ATCGGAA813500.012.440713120
GATCGGA810300.012.387015120
AGATCGG888000.011.351286118-119
TAGATCG220500.011.220171120
CTAGATC53150.09.940381116-117
AAGATCG413900.09.631984120
GAGATCG282400.09.3282120
TTAGATC100150.09.112054116-117
CAGATCG239400.08.4067135120
CACGTCT44150.08.2749405120
ACACGTC46400.07.7355433120
TGAGATC130800.07.34407118-119
Produced by FastQC (version 0.11.5)