FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14731602
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166700.065.47647120
GAGCACA243700.041.496773120
AGAGCAC318950.034.049473120
AAGAGCA490400.021.614084120
GAAGAGC536850.020.4336120
GGAAGAG642400.017.065569120
CGGAAGA750650.014.997314120
TCGGAAG749550.014.643611118-119
ATCGGAA892200.012.771638120
GATCGGA872350.012.613648116-117
AGATCGG966550.011.430385118-119
CTAGATC52600.011.198639110-111
TAGATCG176150.010.790261112-113
AAGATCG514650.09.698335120
TTAGATC81950.08.775888108-109
GAGATCG337450.08.6349120
TAAGATC195750.08.33591120
GCTAGAT25200.07.6104445110-111
CCTAGAT19100.07.530754110-111
TGAGATC164250.07.464461118-119
Produced by FastQC (version 0.11.5)