FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14234564
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164700.064.942406120
GAGCACA250400.040.795902120
AGAGCAC334050.033.65814120
AAGAGCA513750.021.266947118-119
GAAGAGC549350.019.828118-119
GGAAGAG688000.016.32289120
TCGGAAG786250.014.11274118-119
CGGAAGA810550.013.697881118-119
ATCGGAA963500.012.175296120
GATCGGA964200.011.837036118-119
ACACGTC53050.011.201189120
AGATCGG1045700.011.192685120
TAGATCG234550.010.588841118-119
CACACGT50850.010.504091120
CACGTCT54500.010.412701118-119
CTAGATC57500.09.752864116-117
TTAGATC107450.09.164876118-119
AAGATCG478850.08.797323116-117
ACGTCTG71650.08.293413120
GCACACG75550.08.130417120
Produced by FastQC (version 0.11.5)