FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13298378
Sequences flagged as poor quality0
Sequence length60-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACC85350.014.5516371
GAATACC128300.012.86031
GTTGCAT95650.012.1064651
GATGCAT93450.011.7494311
GATTATC169100.011.6024361
GTATACC61000.011.1145951
GTTTACC79500.011.0941811
CATTACC94200.010.827871
GATTACT88200.010.6120941
GTGGTCG397300.010.557295120
GGTCGCC351500.010.430615120
TGGTCGC362650.010.341567120
GATTGAT146500.09.9111211
GCGTCGG75650.09.835733120
GTTACAC49450.09.7066351
GAACACC95850.09.7025211
GATTCAT115950.09.6764471
GATCACC75950.09.6377821
GATTAGC35550.09.6201151
GTTGCAC60500.09.6196981
Produced by FastQC (version 0.11.5)