FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15831499
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC239050.098.76855120
GAGCACA388200.059.741997120
AGAGCAC518550.045.20896118-119
AAGAGCA770500.030.567152118-119
GAAGAGC864700.027.10642118-119
GGAAGAG1046450.022.40268116-117
CGGAAGA1208100.019.502148116-117
TCGGAAG1238700.018.858053114-115
ATCGGAA1445900.016.390263114-115
GATCGGA1470150.016.006445112-113
CACGTCT66350.015.779069120
AGATCGG1608200.014.689721112-113
CACACGT64000.014.264575120
ACACGTC69350.014.13031120
TAGATCG387050.013.320116110-111
CTAGATC77500.012.267079110-111
CGATCTA19900.011.9760525
CAGATCG518600.011.831018110-111
GAGATCG550650.011.7584715110-111
AAGATCG520850.011.755787110-111
Produced by FastQC (version 0.11.5)