FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13903763
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC146400.064.875374120
GAGCACA234500.041.337822120
AGAGCAC299300.032.212334118-119
AAGAGCA471100.021.034344120
GAAGAGC498950.019.5978118-119
GGAAGAG616500.017.048237120
TCGGAAG717550.014.423897118-119
CGGAAGA718500.014.355288120
ATCGGAA864100.012.442922120
GATCGGA856500.012.263521120
AGATCGG942400.011.7695265120
TAGATCG232800.010.718572120
CTAGATC59550.09.707424118-119
TTAGATC102100.09.532725120
AAGATCG413250.09.041192118-119
GAGATCG323750.08.575031120
CAGATCG307250.07.738664120
TAAGATC168400.06.8657455120
CCTAGAT22600.06.792313110-111
TCTAGAT38650.06.591308120
Produced by FastQC (version 0.11.5)