FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11911525
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC92350.060.155853120
GAGCACA151500.037.8229120
AGAGCAC195100.030.522205120
AAGAGCA306250.019.271137118-119
GAAGAGC359800.017.574099120
GGAAGAG458250.014.111789120
TCGGAAG493250.013.819118120
GATCGGA573000.012.996027120
ATCGGAA572250.012.511298120
CGGAAGA587700.011.99121120
TAGATCG133900.011.60763120
AGATCGG643350.011.371092118-119
CTAGATC39450.010.759366120
AAGATCG312750.09.260735120
TTAGATC63550.08.987243116-117
GAGATCG248800.08.555173120
TAAGATC108000.07.8313646118-119
TCTAGAT24950.06.754841118-119
CCTAGAT18650.06.69379116-117
AGAGATC81600.06.655089120
Produced by FastQC (version 0.11.5)