FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15101569
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC209300.098.16465120
GAGCACA323950.063.264843120
AGAGCAC443750.046.06068118-119
AAGAGCA659600.031.161877118-119
GAAGAGC752000.027.133625116-117
GGAAGAG954800.021.686998116-117
CGGAAGA1116400.018.654202114-115
TCGGAAG1135900.018.229029114-115
ACACGTC90500.016.694483120
CACGTCT88600.016.47444120
ATCGGAA1333700.015.920867112-113
GATCGGA1362050.015.489472112-113
AGATCGG1456950.014.483393110-111
CACACGT65150.013.788119118-119
CGTCTGA113550.013.756634120
TAGATCG477550.013.45144112-113
CTAGATC92150.012.888576108-109
ACGTCTG104700.012.645927118-119
CAGATCG725300.012.202052110-111
CTGAACT108950.011.752013120
Produced by FastQC (version 0.11.5)