FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16294155
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC183150.074.718765120
GAGCACA287250.044.74966120
AGAGCAC389400.034.232185118-119
AAGAGCA613300.022.401354120
GAAGAGC665350.020.425236118-119
GGAAGAG817450.017.228043120
CGGAAGA954200.014.9854355120
TCGGAAG965900.014.873814120
ATCGGAA1148900.012.68656118-119
GATCGGA1145550.012.579273118-119
CACACGT48900.011.735265120
AGATCGG1258200.011.584726120
TAGATCG289550.010.678395114-115
AAGATCG579450.09.984991120
CTAGATC76400.09.631781118-119
GAGATCG379650.09.0154915116-117
TTAGATC143300.08.550652112-113
ACACGTC53050.08.526528120
CAGATCG353150.08.325168114-115
CACGTCT55800.07.985325120
Produced by FastQC (version 0.11.5)