FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12953394
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139400.068.07133120
GAGCACA215500.043.730515120
AGAGCAC286750.033.50143120
AAGAGCA443500.022.690094120
GAAGAGC498100.019.766869118-119
GGAAGAG627850.016.339428120
TCGGAAG734550.014.14355120
CGGAAGA744750.014.01114120
ATCGGAA863000.012.431374120
GATCGGA866250.012.215261118-119
CTAGATC55800.011.220212120
TTAGATC98800.011.1556225120
TAGATCG223050.011.110801120
AGATCGG947850.011.081242118-119
AAGATCG429800.09.5747385120
GAGATCG317950.08.820102120
CAGATCG285250.07.542319118-119
TCTAGAT32100.07.50336118-119
TAAGATC173750.07.469513120
TGAGATC150300.07.3331695120
Produced by FastQC (version 0.11.5)