FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477208
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC180250.072.130554120
GAGCACA265950.045.63149120
AGAGCAC363500.035.95972120
AAGAGCA555800.024.209068120
GAAGAGC617700.021.444761118-119
GGAAGAG743450.017.981882118-119
CGGAAGA880200.015.188334116-117
TCGGAAG878650.015.123734116-117
ATCGGAA1040150.012.90314114-115
GATCGGA1065450.012.576412120
AGATCGG1165450.011.446967112-113
TAGATCG242500.010.611157112-113
CTAGATC63600.010.375411110-111
ACACGTC59900.010.374761120
CACACGT53900.09.715997120
TTAGATC94500.09.273613112-113
CACGTCT56950.08.827824120
AAGATCG454050.08.566188116-117
CCTAGAT20750.08.412458108-109
GAGATCG443900.08.298116-117
Produced by FastQC (version 0.11.5)