FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21195569
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG301250.08.324801120
GTGTAGA319600.07.997006120
AGATCTC247600.07.82666120
AGAGTGT359200.07.7834864120
CGTGTAG702250.07.783076120
GAGTGTA323450.07.781251118-119
GTAGGGA572350.07.6941204120
TAGATCT225100.07.4895453118-119
TAGGGAA559150.07.4680204120
TGTAGGG608100.07.3897786120
AAGAGTG419600.07.306511120
GTCGTGT783550.07.2281957120
GTAGATC329200.07.1077046120
GTGTAGG668000.07.0504656120
TCGTGTA755500.06.932732120
CGTCGTG873250.06.884204120
TCTCGGT212500.06.6913543120
GTGGTCG164600.06.5609584120
GGGAAAG610200.06.5286064120
ATCTCGG240750.06.454433120
Produced by FastQC (version 0.11.5)