FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21195569
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178300.034.50769120
GAGCACA239950.026.927622120
AGAGCAC234700.025.63679120
AAGAGCA436500.015.565933120
GAAGAGC434250.014.744171118-119
TCGGAAG569800.012.790854120
CACGTCT78300.012.45281120
ACACGTC89650.012.046471120
GGAAGAG548350.011.884424118-119
CACACGT88150.011.691393120
CGGAAGA648200.010.682082120
GATCGGA798750.010.468887118-119
ATCGGAA732300.010.21376120
AGATCGG862650.010.151258120
TAGATCG213950.09.460926120
CTAGATC48400.08.861588114-115
ACGTCTG95800.08.245492118-119
GCACACG130950.08.035094118-119
CGTCTGA122900.07.6826606120
GAGATCG407750.06.689604120
Produced by FastQC (version 0.11.5)