FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13003500
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1769750.016.271362120
GCGTCGG85100.012.83029120
ATCATTA719750.010.277228120
CATTAAA603500.010.159405120
TCATTAA637650.010.123358120
CCGATCT50400.09.9998124
TCCGATC52700.09.9044043
GTATCAT839800.09.865349120
CCGTATC993400.09.777264120
CCCCCTA8851.0750027E-69.4905811
CGCCGTA1109400.09.360597120
CGTATCA887500.09.281044120
GCCGTAT1056250.09.070533120
TCTCGGG34050.08.985613120
TATCATT830100.08.968834120
ATTAAAA651750.08.864206120
TTAAAAA610500.08.509948120
GTCGCCG1301950.08.492307120
GGTCGCC1370600.08.40836120
TGGTCGC1457250.08.311834120
Produced by FastQC (version 0.11.5)