FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13003500
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC195600.0109.01727120
GAGCACA320450.066.76167120
AGAGCAC432950.049.122032118-119
AAGAGCA640950.032.914074120
GAAGAGC741100.028.603235116-117
GGAAGAG907300.023.190071116-117
CGGAAGA1065200.020.002975114-115
TCGGAAG1091150.019.254602114-115
ATCGGAA1239850.017.259218112-113
GATCGGA1270550.016.57015112-113
TCCGATC53700.015.8522983
CCGATCT52650.015.6724774
CACGTCT63750.015.509825120
AGATCGG1379800.015.470305110-111
CTAGATC64100.014.94756110-111
CCTAGAT18300.014.58354110-111
TAGATCG340150.014.09733114-115
GAGATCG519450.013.307533110-111
CAGATCG476050.012.949567114-115
CACACGT58700.012.819727118-119
Produced by FastQC (version 0.11.5)