FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973477
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139850.072.22519120
GAGCACA222300.046.334120
AGAGCAC305250.034.787933120
AAGAGCA466550.023.259241120
GAAGAGC546500.019.83773118-119
GGAAGAG648800.017.186548120
TCGGAAG779600.014.959353120
CGGAAGA767400.014.738228120
ATCGGAA910650.013.142251120
GATCGGA934450.012.608044118-119
AGATCGG1040000.011.405123118-119
TAGATCG233800.011.084795120
CTAGATC61150.010.486388118-119
TTAGATC91950.010.261845116-117
ACACGTC51900.09.346802120
CACACGT42300.09.268708120
CCTAGAT20550.09.21543114-115
AAGATCG393800.09.0616255120
GGCCTAG3704.8502683E-48.979473112-113
GAGATCG412900.08.794965116-117
Produced by FastQC (version 0.11.5)