FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14188990
Sequences flagged as poor quality0
Sequence length51-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1581100.011.415852120
CGTATCA536100.09.513995120
CCGTATC613100.09.151033120
GTATCAT562100.08.742992120
GCGTCGG91300.08.544023120
TGGTCGC890250.08.47927120
GCCGTAT665050.08.472297120
TCGCCGT806500.08.422322120
GTGGTCG962900.08.2882120
GGTGGTC1032550.08.199847120
GGTCGCC845800.08.187059120
CGCCGTA705350.08.141362120
CGGTGGT1131350.07.9027653120
TCTCGGG33050.07.625488120
TCATTAA542400.07.622358120
TCGGTGG1194150.07.59771120
GTCGCCG850000.07.5079904118-119
CTCGGTG1275050.07.322716120
ATCATTA597150.07.1345196120
TCTCGGT1350450.07.096043120
Produced by FastQC (version 0.11.5)