FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14188990
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC174000.088.58631120
GAGCACA288250.054.307575120
AGAGCAC389600.042.086998120
AAGAGCA599650.027.001066118-119
GAAGAGC668250.024.290306120
GGAAGAG795400.020.23338118-119
CGGAAGA926000.017.412607116-117
TCGGAAG958150.016.727077114-115
ATCGGAA1120950.014.45173114-115
GATCGGA1164900.013.822533112-113
AGATCGG1281100.012.674232112-113
CACACGT45250.012.272728118-119
ACACGTC54800.012.187788120
CACGTCT51700.012.047668120
TAGATCG347550.011.855034112-113
CTAGATC92750.010.883058110-111
GAGATCG478650.010.191615112-113
TTAGATC128100.09.95961110-111
ACGTCTG61450.09.769752120
CAGATCG429650.09.720596110-111
Produced by FastQC (version 0.11.5)