FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15690518
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167550.067.5695120
GAGCACA258850.042.955338120
AGAGCAC339100.033.84806120
AAGAGCA536500.022.318348120
GAAGAGC585400.020.121025118-119
GGAAGAG699650.017.18859120
CGGAAGA823600.015.235569120
TCGGAAG837650.015.0735120
ATCGGAA1003450.012.624325118-119
GATCGGA1004900.012.48683120
AGATCGG1109900.011.337488116-117
TAGATCG277400.011.290951120
CTAGATC63500.010.769608118-119
AAGATCG353150.08.795155120
TTAGATC115450.08.772323114-115
GAGATCG482950.08.685149116-117
CAGATCG385600.07.7950516118-119
ATAGATC137650.07.158078120
CCTAGAT25250.07.093419118-119
TGAGATC190000.07.006035120
Produced by FastQC (version 0.11.5)