FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13380246
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163400.065.75997120
GAGCACA239650.043.26424120
AGAGCAC313100.033.662075118-119
AAGAGCA493700.023.083237120
GAAGAGC534600.020.548122120
GGAAGAG671500.016.690607118-119
TCGGAAG764300.014.592399116-117
CGGAAGA797200.013.93424120
CACGTCT52750.012.861165120
ATCGGAA926000.011.99984114-115
GATCGGA942200.011.971713114-115
ACACGTC57250.011.371446120
AGATCGG1015150.011.131611120
TAGATCG246550.010.631536112-113
CACACGT53850.010.435079118-119
CTAGATC64100.09.94243120
ACGTCTG70500.08.942651120
TTAGATC109250.08.718884120
AAGATCG429500.08.312738116-117
GAGATCG372400.08.299204112-113
Produced by FastQC (version 0.11.5)