FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14965322
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG88650.012.317932120
TGGTCGC434200.09.051002120
GTGGTCG474350.08.967936120
GGTCGCC419100.08.790141120
GATCTCG821300.08.627665120
CTCGGTG664600.08.368827120
TCGGTGG613650.08.242369120
GGTGGTC522100.08.228193120
TCTCGGT696250.08.177982120
CGGTGGT596150.08.162261120
AGATCTC848150.08.0362015120
TAGATCT884250.07.8506103118-119
ATCTCGG753700.07.8491673120
CGCCGTA381800.07.841692120
GTGTAGA1130750.07.837166120
AAAAAAA945000.07.8221126120
AGTGTAG1183600.07.7001286120
TCTCGGG33800.07.633029120
CCGTATC343800.07.6089687120
GTAGATC1130300.07.5801816120
Produced by FastQC (version 0.11.5)