FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11913592
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC262750.052.635002120
AGAGCAC325650.039.122437120
GAGCACA365650.038.593678120
AAGAGCA473400.026.461954118-119
CGTCTGA104500.025.666525120
ACGTCTG108650.024.125116120
CACGTCT110800.023.794525120
GAAGAGC526400.023.54434118-119
TCTGAAC102200.022.218058120
GTCTGAA114000.021.990332120
GGAAGAG626600.019.75532116-117
ACACGTC129550.019.557016118-119
CACACGT135500.019.175901120
CGGAAGA725050.017.29357116-117
TCGGAAG738400.016.68675114-115
GCACACG166500.015.78867120
ATCGGAA889850.014.471827114-115
GATCGGA1135800.012.7335114-115
AGATCGG1246400.011.757254112-113
CTAGATC69050.011.0914112-113
Produced by FastQC (version 0.11.5)