FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14776263
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC208900.0105.57243120
GAGCACA358950.061.557034120
AGAGCAC500950.044.709003120
AAGAGCA740800.030.38494118-119
GAAGAGC820000.027.011576116-117
GGAAGAG960400.023.358847116-117
CGGAAGA1137400.019.577826114-115
TCGGAAG1186300.018.862467114-115
ATCGGAA1387600.016.125734112-113
GATCGGA1402400.016.036062112-113
AGATCGG1534100.014.509395110-111
CACGTCT49600.014.332307120
TAGATCG395450.013.704471112-113
CTAGATC92750.013.435348110-111
CACACGT39950.012.8742895118-119
GAGATCG562750.012.387014110-111
CAGATCG512150.012.068002114-115
TCCGATC62700.012.0337653
TTAGATC144850.011.922794110-111
ACACGTC49400.011.342968120
Produced by FastQC (version 0.11.5)