FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14217068
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152700.079.704834120
GAGCACA246500.052.42937120
AGAGCAC335200.037.648903120
AAGAGCA523150.024.783112120
GAAGAGC605950.022.194582120
GGAAGAG729950.018.189768118-119
CGGAAGA875950.015.858078120
TCGGAAG880300.015.403074120
ATCGGAA1033500.013.694597120
GATCGGA1040500.013.290303118-119
AGATCGG1142450.012.4611635120
TAGATCG294850.011.410055116-117
CTAGATC77250.010.608688118-119
CCTAGAT26050.09.8109665120
TTAGATC113150.09.648174112-113
GAGATCG393950.09.38866112-113
AAGATCG385850.09.334616118-119
GCTAGAT27750.09.307621112-113
CAGATCG400150.09.304301120
CTCTAGA12652.2480272E-68.190653120
Produced by FastQC (version 0.11.5)