FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11666842
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC106550.050.561207120
GAGCACA149250.037.276497120
AGAGCAC189900.030.457039120
AAGAGCA319600.019.219416120
GAAGAGC332250.017.986906118-119
GGAAGAG439850.013.929675118-119
TCGGAAG492150.012.877428120
CGGAAGA520200.012.255647120
GATCGGA582250.011.954812120
AGATCGG617850.010.858537120
ATCGGAA602150.010.696954118-119
TAGATCG160000.010.148384120
CTAGATC42650.09.591609120
CACACGT40450.09.335334120
ACACGTC36000.08.741071120
AAGATCG288850.08.136548118-119
TTAGATC73550.08.074025118-119
GAGATCG201500.07.9837313118-119
CACGTCT35600.07.248214120
CCTAGAT17601.4042598E-97.1517854120
Produced by FastQC (version 0.11.5)