FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14749251
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG24450.028.947151120
TCTAGGT24000.024.574924120
CTAGGTG17600.020.863512114-115
CTAGGGA15000.020.259453108-109
CGTCTAG27300.017.043415120
GTCTAGG20450.015.807316114-115
TCTAGGG21050.015.687151116-117
AAAAAAA1731200.015.372577120
ATCTAGG25500.014.646066112-113
GATCTAG26900.013.767146112-113
GTCTAGA28800.013.403767114-115
CGTAGTG57800.012.131567120
CTAGGGC7800.011.919619116-117
CGAGTAG48950.011.668982114-115
GAGTAGG50700.011.635065116-117
TAGCTCT52150.011.560975120
GTAGGGT30800.011.15324110-111
GAGTCTA33300.010.9327135112-113
TAGGTGG35800.010.656703116-117
GTGTAGC67450.010.411262110-111
Produced by FastQC (version 0.11.5)