FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11313268
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC88650.067.46357120
GAGCACA138550.042.697796120
AGAGCAC185700.032.52033120
AAGAGCA324450.020.57239120
AGTCTAG10200.018.39451490-91
GAAGAGC359800.018.24469120
GTCTAGG11200.016.31805692-93
ACCCGAG11500.015.48557246-47
CCTAGTC6750.014.895156110-111
CGACTAA14350.013.8284874
GGAAGAG485500.013.767585118-119
ACCCCCC15400.013.64608842-43
TCGGAAG491800.013.54506118-119
CCGACTA16100.013.4129253
CCCCCCC33850.012.33073944-45
CGGAAGA568800.012.20229120
ATCGGAA596450.011.33211120
GATCGGA606300.011.024378116-117
AGATCGG637750.010.526489116-117
CCCCGAA45700.09.8338184
Produced by FastQC (version 0.11.5)