FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11545049
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2185550.015.7619915120
CCCCCCC3400.002614192212.3532821
TCATTAA762650.010.611954120
CATTAAA740000.010.312502120
CGTATCA1033500.010.222509120
GTATCAT985900.010.210988120
ATCATTA848950.010.112678120
CCGTATC1141900.010.076951120
TCTCGGG16850.09.969348120
GCCGTAT1229500.09.4419365120
TATCATT987550.09.130795120
CGCCGTA1314550.09.104884120
TCGCCGT1437650.09.005481120
GTCGCCG1529800.08.737539120
ATTAAAA803550.08.712977120
GCGTCGG45200.08.627473120
TTAAAAA789850.08.431154120
TGGTCGC1748550.08.395848120
GGTCGCC1623950.08.245749120
TAAAAAA835150.07.9882120
Produced by FastQC (version 0.11.5)