FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13057468
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC176300.073.11398120
GAGCACA279750.047.073753120
AGAGCAC373150.034.9997118-119
AAGAGCA557700.023.990942120
GAAGAGC623750.021.068815120
GGAAGAG749800.018.034933120
CGGAAGA873000.015.754711120
TCGGAAG892850.015.210284118-119
ATCGGAA1049400.013.637922120
GATCGGA1084700.012.877501118-119
AGATCGG1208850.011.661881118-119
CACGTCT62000.010.642008120
TAGATCG259400.010.51521120
CTAGATC65900.010.064181116-117
CGCTAGA10251.3444514E-79.954306120
CACACGT59250.09.873103120
AAGATCG513300.09.793377116-117
TTAGATC101850.09.750691120
GAGATCG383900.09.444526114-115
CAGATCG354300.08.965805120
Produced by FastQC (version 0.11.5)