FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14579350
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166850.075.434746120
GAGCACA260750.046.435528120
AGAGCAC345000.036.361458120
AAGAGCA540050.023.677956120
GAAGAGC600550.021.145266118-119
GGAAGAG755700.017.077019120
TCGGAAG873450.015.1795435120
CGGAAGA873500.014.766082120
ATCGGAA1041450.012.744188120
GATCGGA1051550.012.693869118-119
AGATCGG1145200.011.934587120
CTAGATC63300.011.93451120
TAGATCG312900.011.363016120
TTAGATC99500.09.821501120
CACGTCT51250.09.466425120
GAGATCG447900.09.299834120
CAGATCG450900.09.114991120
ACACGTC52500.08.448945120
CACACGT48450.08.270866118-119
ATAGATC181800.08.113904116-117
Produced by FastQC (version 0.11.5)