FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14065295
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164400.067.770035120
GAGCACA250400.044.680553120
AGAGCAC338600.034.320377120
AAGAGCA518700.022.305136118-119
GAAGAGC578350.019.885897120
GGAAGAG715050.016.857212120
TCGGAAG839500.014.29616118-119
CGGAAGA845950.014.107074120
ATCGGAA989700.012.428162120
GATCGGA995650.012.174425116-117
AGATCGG1093650.011.220012118-119
CTAGATC61200.010.337507120
TAGATCG254600.010.232545110-111
TTAGATC99000.09.028832114-115
AAGATCG437950.09.013474116-117
CACACGT48550.08.915952120
GAGATCG384150.08.737221120
CACGTCT56950.08.536361120
CCTAGAT20200.08.241993120
CAGATCG347500.08.099217114-115
Produced by FastQC (version 0.11.5)