FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14243053
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA316200.010.284072120
TAGATCT202650.010.154891120
AGTGTAG291450.09.83992120
GTGTAGA291250.09.187485120
AGAGTGT350000.09.085222120
TAGGGAA596500.09.002027120
AAGAGTG408400.08.667502120
GTAGGGA626750.08.634555120
TGTAGGG676250.08.419507120
TCTCGGT182450.08.418289120
AGATCTC216650.08.39096120
GTAGATC303500.08.3422165120
GTGTAGG743850.08.122165120
TCGTGTA848400.07.941578120
GTCGTGT897050.07.8452916120
TGTAGAT330850.07.815807120
GGGAAAG598750.07.785799120
AAAGAGT493550.07.6948524120
CGTGTAG789650.07.620685120
GATCTCG236250.07.5678434120
Produced by FastQC (version 0.11.5)