FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14243053
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139950.055.66498120
GAGCACA200550.040.581707120
AGAGCAC268300.032.43515120
AAGAGCA439550.020.605236120
GAAGAGC479000.019.027212120
GGAAGAG591700.015.820592120
TCGGAAG691150.014.057333120
CGGAAGA714800.013.946654120
GATCGGA839050.012.447515120
ATCGGAA838000.012.334626120
AGATCGG922400.011.439461120
TAGATCG230050.011.177731120
TTAGATC99700.09.78315118-119
CTAGATC64650.09.747825118-119
AAGATCG435800.08.90888120
GAGATCG343300.08.154519120
CCTAGAT22600.07.8469243120
GCTAGAT28350.07.484079112-113
TCTAGAT31850.07.4571857118-119
TAAGATC168650.07.435817120
Produced by FastQC (version 0.11.5)