FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14460366
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193200.080.80311120
GAGCACA310350.049.16666120
AGAGCAC421950.036.83395120
AAGAGCA650450.024.94571120
GAAGAGC703900.022.619694118-119
GGAAGAG864700.018.481226118-119
CGGAAGA1021500.015.681391116-117
TCGGAAG1049200.015.20155116-117
ATCGGAA1251500.013.202528120
GATCGGA1267400.012.944243120
AGATCGG1383050.012.076613120
TAGATCG302950.011.058534120
ACACGTC52250.010.57622120
CTAGATC75950.010.505073110-111
AAGATCG656050.010.276367118-119
CACGTCT53800.010.14312120
CACACGT49300.09.807941118-119
GAGATCG393750.09.622287116-117
TTAGATC143550.09.287117120
ACGTCTG73850.08.885868120
Produced by FastQC (version 0.11.5)