FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15771372
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC200600.079.16696120
GAGCACA311000.048.67278120
AGAGCAC421050.036.965828118-119
AAGAGCA640650.024.929749120
GAAGAGC719250.021.755442118-119
GGAAGAG885700.018.88766120
TCGGAAG1064400.015.729707120
CGGAAGA1046800.015.414641118-119
ATCGGAA1265200.013.403535120
GATCGGA1293750.013.042638118-119
AGATCGG1424750.012.058644120
TAGATCG340450.011.152055118-119
CTAGATC81100.010.635947118-119
TTAGATC115550.09.984512120
CACGTCT52600.09.513393120
AAGATCG467750.09.07976118-119
GAGATCG524900.09.028516116-117
CAGATCG524250.08.940591116-117
ACACGTC55550.08.2575120
CACACGT53800.08.241862118-119
Produced by FastQC (version 0.11.5)