FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18559665
Sequences flagged as poor quality0
Sequence length56-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG159450.010.685963120
GTAGGGA362450.010.295848120
TAGGGAA345600.09.929836120
CGTGTAG489000.09.5698595120
TGTAGGG404700.09.44335120
GAGTGTA178400.09.281839120
AGAGTGT206400.09.127247120
GTGTAGG456400.08.912589120
GTGTAGA182650.08.737391120
TAGATCT114200.08.668375120
AAGAGTG243500.08.52505120
GTCGTGT563650.08.494018120
TCGTGTA526250.08.436444120
AGCGTCG759200.07.886679120
CGTCGTG660350.07.677191120
GTAGATC193000.07.5227714120
AGGGAAA454350.07.368235120
AGAGCGT870150.07.3292403120
GCGTCGT704500.07.3238134120
GGGAAAG406700.07.2578964120
Produced by FastQC (version 0.11.5)