FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12876530
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC175600.090.2096120
GAGCACA286450.056.198986120
AGAGCAC390450.041.080338118-119
AAGAGCA589300.027.62948120
GAAGAGC664400.024.384645120
GGAAGAG836650.019.838978120
CGGAAGA1006750.016.443628118-119
TCGGAAG1017700.016.219728118-119
CACGTCT46900.014.896434120
ATCGGAA1193000.014.030184120
GATCGGA1224300.013.524812116-117
AGATCGG1328100.012.550722114-115
TAGATCG241400.011.485534116-117
CTAGATC58250.010.6685295114-115
ACACGTC49400.010.644441118-119
AAGATCG610100.010.463151114-115
GAGATCG401950.010.227545120
CACACGT49250.010.079554118-119
TTAGATC102700.010.061275116-117
CAGATCG397250.09.599132116-117
Produced by FastQC (version 0.11.5)