FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12660450
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151200.074.37627120
GAGCACA236100.044.924744120
AGAGCAC313150.036.319603120
AAGAGCA476250.023.627909120
GAAGAGC523950.021.515781118-119
GGAAGAG621350.017.834862116-117
CGGAAGA726050.015.649082116-117
TCGGAAG729950.015.259065118-119
ATCGGAA859250.013.2891865114-115
GATCGGA872400.012.832693116-117
CACACGT40400.012.125475120
AGATCGG976350.011.720593112-113
TAGATCG223950.010.901073114-115
CTAGATC61450.09.9896345112-113
CACGTCT44950.09.551805118-119
ACGTCTG59000.09.506181120
GAGATCG435450.09.240692118-119
TTAGATC79950.08.875604110-111
ACACGTC49750.08.847685120
CAGATCG380550.08.382497112-113
Produced by FastQC (version 0.11.5)